GLS2 and hepatocellular carcinoma: The down-regulation of AKT activities by GLS2 was also observed in HCC xenograft tumors; results from both Western-blot and IHC staining assays showed that AKT phosphorylation at Ser473 was clearly reduced in xenograft tumors formed by Huh1 cells with ectopic expression of GLS2 compared with tumors formed by control cells (Figure 4B).