GLS2 and hepatocellular carcinoma: Notably, 5-Aza-dC greatly induced the mRNA levels of GLS2 in these 4 HCC cell lines (by 3-7 folds) as measured by Taqman real-time PCR assays (Figure 7E), indicating that demethylation of the GLS2 promoter increased GLS2 expression in HCC cells.