In contrast, in 110 HCC samples in the TMA, 96 HCC samples showed negative for GLS2 (-; 0% positive staining cells), and 10 samples showed weak staining for GLS2 (±), whereas only 4 HCC samples showed positive staining for GLS2 (+) (p<0.0001; HCCs vs. non-tumor liver tissues). This evidence concerns the gene GLS2 and hepatocellular carcinoma.