We also analyzed pre- and post-synaptic puncta at the inner ML and observed an overall decrease in the number of vGlut [F(1, 16) = 180.00, p < 0.001; −1.98-fold decrease] and PSD95-positive puncta [F(1, 16) = 44.13, p < 0.001; −1.63-fold decrease] in 3xTg-AD mice. The gene discussed is DLG4; the disease is Alzheimer disease.