In the present study, we have investigated the promoter methylation status of candidate tumor suppressor genes namely RASSF1A, APC, MGMT, CDKN2A and CDKN2B in normal bladder mucosa, invasive high grade and non-invasive low grade urothelial bladder cancer using formalin fixed paraffin embedded tissue and plasma samples. This evidence concerns the gene CDKN2A and bladder transitional cell carcinoma.