To determine whether SsnB-induced cell death is mediated by caspase activation, we performed Western blotting as well as immunofluorescence assay to detect active form of caspase-3 in neuroblastoma cells treated with SsnB for 2 days (SH-SY5Y and IMR-32 cells), 3 days (NGP cells) or 4 days (SKNF-1 and SK-N-BE(2) cells). This evidence concerns the gene CASP3 and neuroblastoma.