To understand the mechanism that underlies the alleviation of DSS-induced colitis in mice after treatment with the T. spiralis AES, we examined the levels of some typical Th1 or pro-inflammatory cytokines (IFN-γ and IL-6), Th2 cytokines (IL-4 and IL-13), a Th17 cytokine (IL-17) and regulatory cytokines (IL-10 and TGF-β) secreted by spleen, MLN and colon lymphocytes from differently treated mice using ELISPOT or ELISA. Here, IL4 is linked to colitis.