In the present study, Hcy injection of rats with colitis simultaneously resulted in increased EB content and TNF-α, IL-1β, IL-6 levels as compared with the colitis control group, suggesting that Hcy can promote the release of pro-inflammatory factors in the colonic tissues of rats with colitis, increase intestinal mucosal permeability, and further aggravate intestinal inflammation. This evidence concerns the gene TNF and inflammation.