We screened a total of 77 individuals with a Dutch Lipid Clinic Network score of ≥3, corresponding to possible, probable or definite FH diagnosis [3], and targeted pyrosequencing of the exons and exon–intron boundaries was performed in the LDLR, PCSK9 and LDLRAP1 genes and in APOB exons 26 and 29, including the LDLR-binding domain. This evidence concerns the gene PCSK9 and familial hyperaldosteronism.