Given that many patients in the current study had clinically significant cancer despite having predicted immunogenic mutations and CD8+ TIL, we asked if there was an association between immunogenic mutation load and expression of PDCD1 or CTLA4. We found that patients with higher numbers of predicted immunogenic mutations had increased expression of not only CD8A but also PDCD1 and CTLA4. Displaying these values in a three-way hive plot (Krzywinski et al. 2012) highlights the association between these T cell markers and immunogenic mutation load (Fig. 4). This evidence concerns the gene CTLA4 and cancer.