Having in mind the conflicting role of CD4lo T cells in T1D, we have additionally analyzed CD4hi/CD4lo panel in our groups of subjects, showing low level of CD4lo subpopulation in lymphocytic gate (hrFDRs: 1.03 ± 0.73%, lrFDRs 1.23 ± 1.06%, RT1D: 0.96 ± 0.57%, HC 1.25 ± 1.52%, P = NS) and among CD4+ T cells (hrFDRs 2.67 ± 0.55%, lrFDRs 3.17 ± 2.66%, RT1D 2.24 ± 1.37%, HC 3.05 ± 3.89%, P = NS), range (0–6.77% and 0–17.32%, resp). Here, CD4 is linked to type 1 diabetes mellitus.