SREBF1 and hepatocellular carcinoma: To investigate the effect of SREBP-1 in HCC cells, we first evaluated mRNA and protein levels in LO2, Hep3B, MHCC97L, Huh7 and HepG2 cells, and found both mRNA and protein were highly expressed in HepG2 and MHCC97L cells compared with the other three cell lines (Figure 3A,B).