In this study, to explore novel interactive insights between Aur A/B and BRCA1/2, we used specific shRNAs to consecutively silence the expressions of Aur A/B and BRCA1/2 in pancreatic and ovarian cancer cell lines, and analyzed the cell proliferation, cell cycle progression, cytokinesis, chromosomal polyploidy, and tumorigenesis of resulting cells. This evidence concerns the gene BRCA1 and ovarian carcinoma.