Diarrhea-associated E. coli strains encoded virulence factors typical for each of the diarrhea-associated pathotypes including EAggEC (pCVD432), ETEC (lt/st), EIEC (ial/ipaH), EPEC (eaeA/bfpA), EHEC (stx1/stx2/ehly) and DAEC (afaI) strains. Here, STX2 is linked to Diarrhea.