Another approach that created a bioscore using PCT, sTREM-1, and CD64 index in 300 consecutive patients and subsequently externally validated the score in an independent prospective cohort of 79 patients found each biomarker to be independent predictors of infection but the performance of the bioscore to be superior to each individual biomarker and significantly elevated (P < 0.001) in patients with sepsis compared to noninfected patients [129]. The gene discussed is CALCA; the disease is Sepsis.