As presented in Fig. 7A TGF-β exposure for 1h did not induce any significant effect on both epithelial and mesenchymal mRNA transcript levels in PCa cell line (22Rv1); however, 24h treatment of TGF-β significantly reduced the mRNA transcripts of epithelial markers i.e. E-cadherin and occuludin, and also subsequently increased the mRNA transcripts of mesenchymal markers i.e. vimentin and snail. Here, SNAI1 is linked to posterior cortical atrophy.