The findings of the present study showed that the promoter region of the APC gene was methylated in 35.09% of the non-cancerous liver tissues and 64.91% of HCC samples using a cut-off set at 4% PMR, which indicates a high methylation frequency of the APC gene promoter in HCC and a moderate methylation frequency in the paired non-tumorous liver tissues. Here, APC is linked to hepatocellular carcinoma.