Taken together, the present study results showed that hyperthermia treatment at 52.5±2.5°C using magnetic nanoparticles inhibited tumor growth, promoted the survival of the tumor-bearing rats and inhibited angiogenesis potentially by the downregulation of the expression of VEGF and its receptors, including Flt-1 and Flk-1. This evidence concerns the gene FLT1 and neoplasm.