We did not find evidence that RAP directly affected the expression of β-catenin/TCF-regulated genes in the cultured HCECs, and the means by which RAP dramatically inhibits Apc-mutation dependent polyposis in mouse colon epithelium in vivo is likely independent of direct effects on the β-catenin-dependent Wnt signaling pathway. The gene discussed is HNF4A; the disease is polyposis.