We used real time primers based on the sequence obtained and observed that A. hydrophila-infection induced a several fold increase in PKACA transcripts with maximum expression recorded by 2–6 h p.i. (P<0.05) (Figure S2B) and thereafter it started declining (data not shown) though significant level of PKACA mRNA expression was noted till 24 h p.i. This decline in PKACA transcripts at later time points probably serves as controlling mechanism to prevent the overshoot of cAMP dependent downstream events in the infected cells. The gene discussed is PRKACA; the disease is infection.