Once acquired through an infected blood meal, the BSF parasites encounter a number of physical and immunological barriers in the gut [9], [10], [11], such as the peritrophic matrix [12] and a battery of host immune molecules, including reactive oxygens (ROS), antimicrobial peptides (AMPs), Peptidoglycan Recognition Proteins (PGRPs), tsetse EP Protein which restrict the establishment of successful infections [12], [13], [14], [15], [16], [17], [18], [19], [20]. Here, PGLYRP1 is linked to infection.