When each Treg subset isolated from HNSCC patients was co-cultured (1:1 ratio) with CD25-CD45RA+CD4+ responder cells, both CD45RA-CD25+++ and CD45RA+CD25++ Tregs consistently induced a greater percentage of suppression compared with CD45RA-CD25++CD4+ T cells (90.34 ± 3.22% vs. 10.81 ± 1.64%, P < 0.001; 88.60 ± 4.86% vs. 10.81 ± 1.64%, P < 0.001, respectively) (Figure 2A-E). The gene discussed is CD4; the disease is head and neck squamous cell carcinoma.