In conclusion, in this paper, we presented experimental evidence which supported that Notch-1 signaling promoted invasive growth of MDA-MB-231 cells, including adhesion, invasion and motility through activating NF-κB pathway, and the expression level of Notch-1 was also associated with the growth and migration of breast cancer MDA-MB-231 cells. This evidence concerns the gene NOTCH1 and breast carcinoma.