To identify their roles in CIS-induced loss of tonic GABAARs currents, we pretreated the CIS mice 30 minutes prior to the daily immobilization with either RU 38486 (20 mg/kg/day), a GR antagonist or spironolactone (100 mg/kg/day), a MR antagonist (Figure 5A). This evidence concerns the gene NR3C1 and in situ carcinoma.