Figure 4D shows significant loss of AQP4, GFAP and MBP immunofluorescence at 5 days after two injections of 75 mg IgG purified from NMO patient serum, which was not seen in rats receiving the same amount of IgG from control (non-NMO) human serum. The gene discussed is AQP4; the disease is neuromyelitis optica.