Interestingly, compound 1 induced both early and late apoptosis death of GBM cells, as well as the transcription induction of the pro-apoptotic gene PUMA, differently from what obserced with the standard MDM2 inhibitor, Nutlin-3, that did not show any induction of specific apoptotic parameters at short-time of treatment (Figure 7)15, 16. Here, BBC3 is linked to glioblastoma.