Among the samples screened for the TJP2 gene, the three individuals with hearing loss who carried the c.3562A>G (p.T1188A) variation were I-2 (age, 62 years) and II-1 (age, 37 years) of the KNUF25 family and II-6 (age, 57 years) of the YS-149 family. The gene discussed is TJP2; the disease is hearing loss disorder.