Fatigued women (Piper Fatigue Scale [PFS] score ≥5) with iron deficiency (ferritin <50 μg/L and transferrin saturation <20%, or ferritin <15 μg/L) and normal or borderline hemoglobin (≥115 g/L) were enrolled in 21 sites in Austria, Germany, Sweden and Switzerland, blinded to the study drug and randomized (computer-generated randomization sequence) to a single FCM (1000 mg iron) or saline (placebo) infusion. This evidence concerns the gene TF and nutritional disorder.