Plasma insulin levels were significantly increased in both young (LF: 0.077±0.19; HF: 0.66±0.09, P≤0.05) and middle-aged mice (LF: 0.18±0.02; HF: 1.34±0.15, P≤0.05) fed a HFD compared to mice fed a LFD. This evidence concerns the gene INS and hydrops fetalis.