The results showed that the levels of S1P associated with diabetic HDL3 were significantly higher than native HDL3, and S1P in diabetic HDL2 and native HDL2 showed no difference (Figure 2B, T2DM-HDL3 vs. control-HDL3: 189.7 ± 7.4 vs 230.9 ± 13.8 ng/mg, p < 0.01). This evidence concerns the gene MBTPS1 and type 2 diabetes mellitus.