BCR and chronic myelogenous leukemia, BCR-ABL1 positive: To elucidate the in vivo expression pattern of Evi1 in CML-CP, 5-fluorouracil (5FU)-primed BM cells from heterozygous Evi1-IRES-GFP (Evi1+/GFP) knock-in mice were retrovirally transduced with BCR–ABL (detected with Kusabira Orange (KuOr) fluorescent protein) and injected into lethally irradiated recipient mice to generate ‘Evi1-reporter' CML-CP mice (Figure 1d).