Further to confirm our primary screen results, we performed a secondary screen of 16 genes identified as negative regulators of TRAIL-induced caspase activation in four cell lines representing different subtypes of breast cancer (TNBC, ER positive, and HER2 amplified) (Figure 5, Table 1, and Additional file 8: Table S4). The gene discussed is TNFSF10; the disease is breast carcinoma.