Similarly, because we studied mRNAlevels from whole artery homogenates, it is unknown whether differences in aortic gene expressionreported in this study are originating from the endothelium, smooth muscle, or adventitia.Examination of the impact of NOS inhibition and obesity on vascular gene expression with separationof cell populations should be a priority in future studies. This evidence concerns the gene NOS1 and obesity due to melanocortin 4 receptor deficiency.