Their results, however, were not replicated in a large study by Agerholm-Larsen et al. that investigated the association between the ACE polymorphism and ischemic heart disease in a case-referent format (n = 10,150) and in a retrospective cohort format (n = 7263); no significant differences in the incidence of myocardial infarction or any other manifestation of ischemic heart disease between the genotype classes were noted [38]. This evidence concerns the gene ACE and coronary artery disorder.