Their in vivo study using hydrodynamic transfection not only exhibited, in line with earlier studies [153, 154], the cooperative role of activated Wnt/β-catenin and Ras in induction of HCC, but also revealed that dominant negative Spry2 cooperates with β-catenin to induce development of liver cancer in mice, with tumor cells showing upregulation of ERK and deregulation of genes involving in cell proliferation, apoptosis, and angiogenesis. Here, SPRY2 is linked to hepatocellular carcinoma.