TGFB1 and esophageal squamous cell carcinoma: [7]–[13] The expression of DACH1 was regulated by promoter region hypermethylation in endometrial, colorectal and hepatocellular cancer. [11]–[13]DACH1 suppressed human hepatocellular carcinoma by activating TGF-β signaling. [13] While the epigenetic changes and the function of DACH1 in human ESCC remain unclear.