As a change in the nucleotide sequence within the gene coding region can alter the expression and/or mRNA stability and thus the final protein concentration, we sequenced a cDNA fragment of 1,259 bp covering the whole ILKAP exons (Chr 2∶239,079,043–239,112,324 according to UCSC Genome Browser, GRCh37/hg19), in order to assess if there were nucleotide changes in the melanoma cell lines. The gene discussed is ILKAP; the disease is melanoma.