ILKAP and melanoma: In the absence of coding region mutations that could be associated to the ILKAP expression deregulation observed in melanoma cell lines, we hypothesized that there could exist mutations in the promoter region that could be altering the transcriptional activity of ILKAP. Therefore, the ILKAP gene promoter region was amplified from genomic DNA using two pairs of primers that resulted in one fragment of 1,443 bp and another overlapping fragment of 1,110 bp.