Although some individual heterogeneity was also observed as not all the melanoma cell lines showed a reduction in ANXA5 or ILKAP gene expression, the trend observed coincided with that obtained previously using expression microarrays, in which only ILKAP, but not ANXA5, showed significant differences at mRNA transcriptional levels between melanocytes and the tumor cell lines studied (unpublished data). This evidence concerns the gene ANXA5 and melanoma.