Thereby, although the results obtained suggest that allele C in rs6854854 has a protective role against melanoma (p-value = 4×10−5), with an OR of 0.541 (95% CI 0.371–0.791; p-value = 0.0013), after genotyping 464 MM patients and 400 disease-free controls, the ANXA5 rs6854854 SNP did not reach statistical significance in validation Phase II. This evidence concerns the gene ANXA5 and melanoma.