CD8A and melanoma: Another important component of successful vaccination with DCs is their ability to stimulate CD8+ T cells, which requires production of IL-12-p70 by DCs, which, in turn, depends on stimulation of DCs by two signals: CD40 or TLR and INF-γ A recent small clinical trial used DCs from peripheral blood mononuclear cells (PBMCs) of melanoma patients pulsed with gp100 peptides.