QRT-PCR experiments in two melanoma cell lines (501mel, WM9) and in cells freshly isolated from a human biopsy (C-09.02) showed, as expected, that MITF suppression by siRNA, compared with control cells, caused a decreased mRNA expression of two of its target genes, MLANA and CDK2 [17] and an increased mRNA expression of CCL2, as previously reported (Figures 5A and S3B-C) [15]. The gene discussed is MITF; the disease is melanoma.