To demonstrate the strong impact of Cx43 on GJIC in pancreatic cancer, we inhibited Cx43expression by siRNA transfection in GJIC-competent BxPc-3 cells, leading to a completelyabrogated GJIC, which was associated with a higher basal viability, resistance togemcitabine, enhanced clonogenic potential and the induction of stem cell-associatedfactors. This evidence concerns the gene GJA1 and pancreatic neoplasm.