CCNE1 and esophageal adenocarcinoma: By immunohistochemical analysis, high expression of cyclin E was observed in 2.3% of normal squamous mucosa (2/86), 3.7% in columnar cell metaplasia (3/81), 5.8% in Barrett’s esophagus (2/34), 19.0% in low grade dysplasia (4/21), and 35.7% in high grade dysplasia (5/14).