Regarding the expression of the negative costimulatory molecules such as PD-1 and PD-L1, we observed a higher expression of PD-1 in patients with more than one invaded lymph node arguing that in melanoma advanced stage an increase in tumor burden was associated with an induced expression of the coinhibitory molecule PD-1 in the T lymphocytes which could explain the loss of efficacy of TILs. The gene discussed is CD274; the disease is melanoma.