The liver and hypothalamus were obtained from the same animals to analyze GK localization in response to fasting condition, normoglycemia and hyperglycemia induced by 16 h fasting or 30 min post-intraperitoneal injection of 2.3 mL of saline or 0.5 g/kg or 4 g/kg body weight glucose, respectively. The gene discussed is GK; the disease is Hyperglycemia.