AR and Familial prostate cancer: Using the androgen-dependent LNCaP cell line and chromatin immunoprecipitation sequencing (ChIPseq), we showed that two potent oncogenes in prostate cancer, the AR (Massie et al, 2011) and c-Myc, bind upstream of the HES6 coding sequence (Supplementary Fig S1A) to positively regulate levels of HES6 mRNA (Fig 1A, Supplementary Fig S1B and C) and confirmed that overexpression of c-Myc overcomes growth inhibition by bicalutamide (Bernard et al, 2003) (Supplementary Fig S1D), an effect that can be reversed by knock-down of HES6 (Supplementary Fig S1E).