For the first time HRCA of the PLB1 locus alone or together with the application of the herein developed semi-nested PCR approach showed to be a specific and highly sensitive methodology, with a great potential to be used on DNA extracts from pure cultures or clinical specimens for the direct identification of the different major molecular types and potential hybrids of the agents of human and animal cryptococcosis, providing the basis for a rapid and patient tailored treatment choice for this disease. Here, PLB1 is linked to cryptococcosis.