Using Luminex technology with the blocking of heterophilic antibody, increased levels of TNF-alpha, IL-1beta, IL-6, IL-12P40, IL-13, and several chemokines (CXCL10, CCL11, CCL2, and IL-8) were observed in sera from RA patients with <6-month symptom duration compared to HC [23]. This evidence concerns the gene TNF and rheumatoid arthritis.