Data from the current study showed that miR-144-3p mimics (agomir) suppress the expression of ABCA1 and enhance inflammatory factors, both in vitro and in vivo, inhibit cholesterol efflux in THP-1 macrophage-derived foam cells and impair RCT in apoE−/− mice fed a high-fat diet (HFD), leading to accelerated pathological progression of atherosclerosis in experimental mice. Here, APOE is linked to atherosclerosis.