To evaluate the effect of BPAF on endogenous transcription, we conducted the real-time PCR to detect mRNA levels of estrogen responsive genes, such as TFF1, GREB1 and CTSD in ERα-positive T47D and MCF7 breast cancer cell lines and ERα-negative MDA-MB-231 breast cancer cell line. Here, GREB1 is linked to breast carcinoma.