AFAP1L1 and colorectal carcinoma: We first analyzed the expression levels of the AFAP1L1 gene by quantitative RT-PCR (qRT-PCR) using frozen tissues from 33 CRC specimens (two cases at Stage I, 11 at Stage IIa, four at Stage IIb, nine at Stage III, and seven at Stage IV, respectively) along with paired adjacent normal colonic mucosa samples.