CYGB and melanoma: The results revealed that all 9 CpG sites were methylated in A375 but were totally unmethylated in melanocytes and G361, demonstrating that the transcriptional inactivation of the promoter by DNA hypermethylation occurred in A375 (Fig. 3) and in other melanoma cell lines in which the expression of the CYGB gene is down-regulated (Fig. S4).