AKT1 and neoplasm: Evaluation of the amount of activated Akt as determined by phosphorylation of both the serine (Ser473) and threonine (Thr308) residues revealed that phosphorylation gradually increased in the WT, Akt1−/− and Akt3−/− mice over time (Figure 6E and F, panels 1, 2 and 4) whereas the amount of activated Akt in the Akt2−/− mice did not change over time (Figure 6E and F, panel 3), despite the fact that tumor growth in these mice was very aggressive (Figure 2G and H).