Immunohistochemical staining of lung tissue sections from AJEJJenv infected WT, Akt1−/−, Akt2−/−, and Akt3−/− mice with a Jenv-specific monoclonal antibody revealed that all tumors uniformly express the viral oncogene suggesting that tumor initiation in this model was dependent on Jenv expression (Figure 4A, D, G and J). Here, AKT3 is linked to neoplasm.