Evaluation of the amount of activated Akt as determined by phosphorylation of both the serine (Ser473) and threonine (Thr308) residues revealed that phosphorylation gradually increased in the WT, Akt1−/− and Akt3−/− mice over time (Figure 6E and F, panels 1, 2 and 4) whereas the amount of activated Akt in the Akt2−/− mice did not change over time (Figure 6E and F, panel 3), despite the fact that tumor growth in these mice was very aggressive (Figure 2G and H). This evidence concerns the gene AKT3 and neoplasm.