To confirm our mass spectrometry results, we studied the expression of PSCA and EEF1α by immunocytochemistry in six head and neck cancer cell lines: 3 HPV+ cell lines (93VU-147T, UPCI-SCC90 and UPCI-SCC154) and 3 HPV− cell lines (FaDU, Detroit and UPCI-SCC131). The gene discussed is EEF1A1; the disease is head and neck cancer.